Lab manual: Coss and Oates. 2000, Immunology Laboratory Exercises, available at the
JLC Bookstore

NOTE:   Lab manual must be brought to the first day of class AND placed into a 3-ring binder.
 

LAB	WEEK OF	EXERCISE
1 2 4,5 7 8,9,10 11,12 13,14 16 17 18 19	Jan.   22 Jan.   28 Feb.    4 Feb.  11 Feb.  18 Feb.  25 Mar.    3 Mar.  10 Mar.   17 Mar.   24 Mar.   31 Apr.     7 Apr.   14 Apr.   21 Apr.   28	BIOL453 labs do not meet this week Introduction and Quality Control - BRING LAB NOTEBOOKS Non-Specific Immunity,  Introduction  to SigmaPlot Leukocyte Differential and Cell Count Protein Determination Agglutination Reactions Precipitation Reactions, RID and *MIDTERM EXAM SPRING BREAK SPE: Serum Protein Electrophoresis  IEP: Immunoelectrophoresis  Flow Cytometery - Lecture ELISA: Enzyme-Linked Immunosorbant Assay SDS-PAGE Western Blot Analysis  *FINAL EXAM

COURSE NOTES:

Occasionally, you and/or your lab partner(s) will be required to work in the laboratory between class meetings for short periods of time. The scheduling of these times will be arranged during the lab period. You are responsible for remembering to return and make your observations.

Attendance and prompt starting times for the Laboratory experiments are very important so that you will hear all the information and precautions the instructor has given. Attendance is mandatory and laboratory experiments cannot be set up at times other than already scheduled. If you are unable to attend your assigned section, you must contact the lab instructor to see if there is room available in the other lab sections.
 

COURSE GOALS:

• To become familiar with equipment used in todays research and clinical laboratories
• To understand the underlying immunological principals of current laboratory techniques, both in medical/clinical and research laboratories
• To be able to analyze and interpret data generated using immunological techniques

GRADING POLICY:

I.   Attendance and completion of each laboratory period is worth 5 points. One point will be deducted from each exercise for each of the following infractions: 1) You are not present at the start of the lab period,  2) you are not prepared for the lab exercise  3) your lab bench is left unclean and/or equipment not put away,  4) any samples not properly labeled, 5) any samples/tests not discarded after the necessary incubation periods and 6) any noncompliance with the safety rules of the laboratory.

II.  Laboratory notebooks will be collected in the middle of the semester without prior notification and again at the end of the semester. A maximum of 40 points may be earned on the notebooks. Include in ALL of your exercises a separate DISCUSSION/CONCLUSION section that includes: 1) the significance of the results, 2) the interpretation of the data, 3) any problems encountered during the exercise and 4) any changes you would make if you were to repeat the experiment. NOTE: Notebooks will not be accepted after the class period in which the notebooks are collected.

III.  Exams and Quizes: There will be four unannounced quizes during the semester, each being worth 5 points. There are no makeup quizes. The midterm and  final will each be worth 40 points.
 

GRADING SCALE:90 -100% = A, 80-89% = B, 70-79% = C, 60-69% = D, < 60% = F
NOTE: In addition, grades of +/- may be used at the discretion of the instructor.

*NOTE:  If an exam is cancelled for any reason (weather, etc.),  the exam will be given during the next class meeting.

All exams must be taken at the scheduled times; there are no makeup exams. A grade of "0" will be recorded for each missed exam or quiz.

The GMU Honor is in effect at all times. Cheating will not be tolerated and it is your duty as a GMU student to report any violations of the Honor Code to your instructor.

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Page last updated Januaury 14, 2008