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Lawrey Lab

 

Current Research Projects - Cyanolichens:

 

 

Approximately 10% of all lichens form associations with cyanobacteria, an ancient and diverse group of photosynthetic bacteria.

As photosynthetic organisms, cyanobacteria are important components of many microbial communities. Since many are also capable of fixing atmospheric N, they provide significant inputs to the global N budget.

Cyanobacterial classification has traditionally been based on morphology, but recent use of molecular data, principally 16S rRNA sequences, is beginning to shed new light on the evolution of the group.

Based on morphological identifications, the most common cyanobacterial photobionts in lichens were assumed to be members of the genera Nostoc, Scytonema, Stigonema, Calothrix, Dichothrix, and Chroococcidiopsis. These estimates were preliminary, however, since very few lichen cyanobacterial photobionts have been isolated in culture or sequenced to confirm identifications.

Recent sequencing studies indicate that morphology is not always a reliable indicator of evolutionary history. In our lab we are investigating the diversity and evolution of cyanobacterial photobionts of cyanolichens, focusing on those assumed to contain Scytonema or Scytonema-like forms, which have never before been sequenced..

These investigations have led to the discovery of a remarkable clade of cyanobacteria that appears to be entirely lichenized. This 'scytonematoid' group is mostly tropical and genetically distinct from all other heterocystous cyanobacteria. In addition, phylogenetically unrelated lichens appear to 'share' the same photobiont types, which suggests an evolutionary history not unlike that observed in crop plants whose history is shaped by domestication.

We are now sampling and sequencing other cyanolichens from the Neotropics, including the Galapagos Islands. These studies are revealing other previously unrecognized groups of lichenized cyanobacteria, and indication that lichenization represents a significant source of biodiversity in this group.

 

 cyano tree

 

 

 

 

Nostoc

 

Scytonema

Scyto in D glabratum

 


 

In collaboration with investigators at several other labs, we have been investigating:

1. The phylogenetic position of scytonematoid photobionts from the Neotropics. Collaboration with R. Lücking, F. Bungartz. J. L. Chavez.

See Lücking, R., J. D. Lawrey, M. Sikaroodi, P. Gillevet, J. L. Chaves, H. J. M. Sipman, F. Bungartz. 2009. Do lichens domesticate photobionts like farmers domesticate crops? Evidence from a previously unrecognized lineage of filamentous cyanobacteria. American Journal of Botany 96: 1409-1418.

 

See also eBioNews story on this project.

2. Specificity and selectivity of cyanolichen fungi and the consequences to cyanobacterial evolution. Collaboration with R. Lücking, F. Bungartz. J. L. Chavez.

3. The phylogenetic distribution of cyanolichens and their photobionts in the Galapagos. Collaboration with R. Lücking and F. Bungartz.