Permanent study sites (presently 102 total) were established in nine NCR park units:
Sites were placed in forested locations where a sample of one lichen species (Flavoparmelia caperata) could be obtained for elemental analysis.
In CATO and PRWI sites were located within 1 km2 grids.
A 20m X 20m quadrat was established at each site and its GPS
Site data (abundance scores)
sampling protocol was similar to that of McCune et al. (1997) used in the
Forest Health Monitoring Program (complete details of the methods are given
in Tallent-Halsell,1994); the only difference is that a plot of 20m X 20m was
used instead of a circular plot of 36.6 m radius.
§ Within each quadrat presence of all corticolous (bark-inhabiting) macrolichen species was recorded; species on dead and down trees were included.
§ Terricolous (ground-dwellling) species and corticolous species below 0.5 m on trees were included as off-frame information.
§ Sensitivity to pollution was assess for species based on a literature survey.
§ The abundance of all lichen species was also recorded using abundance scores from McCune et al. (1997):
1 = rare (< 3 individuals in plot)
Sampling for elemental analysis
A sample of a single lichen (F. caperata) was obtained in each plot for elemental analysis. This species has been used in several previous biomonitoring studies done in the NCR.
Lichen samples were analyzed for metals (Cd,Cu,Pb,Ni,Cr,Zn, EPA method 3051), total sulfur (3051 digest), and Hg (EPA method 7471).
Seven park units (CATO, CHOH, GWMP, NACE, NAMA, PRWI, ROCR) were sampled in 2004; HAFE and MANA were sampled in 2006. All park units were resampled in 2009.
of NCR park units
Flavoparmelia caperata used in elemental analyses. Image © 2006 by Paul Diederich from http://www.lichenology.info.