Welcome to the Grant Lab

The Grant lab is located in Discovery Hall, Rm 254

Prince William Campus,

ggrant1@gmu.edu   Contact: 703-993-4292

Office hours: Monday 2-4pm Discovery Hall,  Rm 207

Online Guide for Bioscience PhD Students.

Courses:
Spring 2008	BIOS 740
Previous courses:	BIOL 484 BIOL 690 BIOS 702 BIOL 690
	BIOL 484 & BIOL 584
	BIOL 691
	BIOL 508 Lab and Lecture series
	BIOL 695 & BIOS 704
Important Links
Great Animations for  Biology	Lab interests and Publications
	Molecular  and Microbiological Department MMB
	The Biology Program (BD)
	Biosciences PhD program (BIOS)

The Grant Lab Interests

The Grant lab is currently funded by the Commonwealth Health Research Board to investigate the dedifferentiation of normal human chondrocytes in tissue culture, and the role of KLF4 and the Wnt pathway in this dedifferentiation.

The Grant lab is also collaborating with

INOVA Lung Transplant & Advanced Lung Disease Program in the investigation of differential gene expression of cell populations in Fibrotic disease of lungs.

Other Grant Lab interests:

• Osteoarthritis
• Investigations into the dedifferentiation of primary cells.

• NIAMS
• Arthritis Foundation
• Cultured Chondrocytes
• Uses of Autologous Chondrocytes

• In vitro Toxicology of  JP8 Jet Fuel.
• Alternatives to Animal Research:   CAAT

Courses:

The honor code is strictly observed in all courses

• BIOL 508 Animal Cell Biotechnology Lecture Series 3 Credits
• BIOL 508 Animal Cell Biotechnology Workshop 3 Credits, Spring 2003
• BIOL 691 Microarray Techniques and Analysis Workshop 3 Credits, Summer 2003
• BIOL 695 Seminar in Molecular, Microbial and Cellular Biology (1:1:0) Fall 2003
• BIOS 704 Topics in Biosciences (1:1:0) Fall 2003
• BIOL 690 Introduction to Graduate Studies (1:1:0) Fall 2006
• BIOL 484 Eukaryotic Cell Biology (3:3:0) Fall 20075
• BIOS 702 Research Methods (3:0:0) Fall 2006
• BIOS 740 Lab Methods in Functional Genomics and Biotechnology. (3:1:6) Spring 2008

Summer 2003

Biol 691: Microarray Techniques and Analysis Workshop

August 11th -15th 2003
At: Prince William Campus PWII
9am - 5pm M-F

Map to GMU's Prince William Campus

This course introduces the student to the topics and practical side of microarray technology.

3 Credit Course Prerequisites:  Biol 485, Or the permission of Instructor

Exam breakdown:

Final exam on Friday the 15^th is 50 % of your grade the remainder is a take home exam due Friday August 22nd at Midnight..

Text: Microarray - Mark Schena
DNA Microarrays: Gene Expression Applications

Other available titles

Lecture will be held in room 203 Prince William II

This course will cover all the aspects of Microarray  wet lab procedures from RNA isolation to hybridization and analysis of data.  The course will run from Monday the 11th of August 2003 to Friday the 15th of August at the Prince William campus.  

For Wednesday August 13th the follow 2 papers are reviewed, please down load and read BEFORE August 13th.

Lectures:

The Magic of Microarrays

Libraries and Surfaces

Targets and Probes

Hybridization

Microarray Detection

Eberwine Paper

Microarray Informatics

PCR

Group Results

Lab coats are required for this course

• Students will learn to extract and quantitate total RNA.
• Amplify RNA and label
• Hybridize with custom cDNA chips
• Scan using ScanArray 5000
• Analyze results

Lectures

• Microarrays and introduction
• RNA Isolation extraction and
• Attachment Chemistries and Printing
• Labeling of sample
• Chip Manufacture, Library manufacture
• Hybridization
• Scanning
• Analysis

Interesting Links

123 Genomics Homepage

The Institute for Genomic Research (TIGR)

Cold Spring Harbor  The Dolan DNA learning Center

NIH website for Science

Spring 2003

Biol 508: Animal Cell Biotechnology Workshop

March 10^th – 15^th 9am – 5pm PWII

This is a 2 Credit course

· Exam Breakdown

· Final exam (50%)

· Term paper (50%)

Lab coats are required for this course

Lecture Topics

Laboratory set up and routine techniques

Media, serum, growth and attachment factors

Primary cell culture

Cytotoxicity assays

Genetic manipulation of cells - Transfection/ Infection

Large Scale cell culture – Hybridoma and Vaccine production.

Tissue Engineering, gene therapy and stem cells

Exam March 15^th pm

Paper due March 28^th Friday

TEXT:

Culture of Animal Cells – A manual of basic techniques. R. Ian Freshney 4^th edition (3^rd is fine also)Wiley-Liss. Publication. ISBN 0-471-34889-9
Library Location: Fenwick Stacks Call Number: QH585.2 .F74 2000

Additional Reading:
Cell and Tissue Culture: Laboratory Procedures in Biotechnology. Alan Doyle and J. Bryan Griffiths.
Wiley Publication. ISBN 0-471-98255-5

Animal Cell Culture Methods. Jennie P. Mather and David Barnes.
Academic Press. ISBN 0-12-544159-2 (hp)

Spring 2002

Biol 508: Animal Cell Biotechnology Lecture Series

3 Credit course

Exam breakdown:

•          1 midterm exam (20%)
•          1 final exam (55%)
•          Term paper (20%)
•          Presentation and class participation (5%)

The course introduces the student to the topic of mammalian cell culture, in vitro toxicology,

gene therapy and industrial application of animal cell biotechnology.

Lecture No	Topic
1	Introduction to mammalian cell culture
2	Definitions
3	Laboratory set up and routine techniques
4	Media and serum.
5	Growth Factors and attachment factors
6	Primary cell culture
7	Exam
	Break
8	Cytotoxicity assays
9	Genetic manipulation of cells - Transfection/ Infection
10	Large Scale cell culture – Hybridoma and Vaccine production.
11	Tissue Engineering
12	Gene therapy
13	Stem cells
14	Cancer
	Exam
	Paper due

TEXT:

Culture of Animal Cells – A manual of basic techniques. R. Ian Freshney 4^th edition (3^rd is fine also)

Wiley-Liss. Publication. ISBN 0-471-34889-9

Library Location: Fenwick Stacks Call Number: QH585.2 .F74 2000

Additional Reading:

Cell and Tissue Culture: Laboratory Procedures in Biotechnology. Alan Doyle and J. Bryan Griffiths.

Wiley Publication. ISBN 0-471-98255-5

Animal Cell Culture Methods. Jennie P. Mather and David Barnes.

Academic Press. ISBN 0-12-544159-2 (hp)

Biol 695 & BIOS 704

Seminar Series




This course 1 credit seminar series is run every second Thursday for the Fall 2003 Semester
 at the Prince William Campus
PWI Rm 203 between 7.20pm and 10pm


Directions to the PW Campus

Class Schedule:  We will meet  8 times:

                                                                                                        August 28th,

                                                                                                        September 11th
                                                                                                        September 25th:   Presenters;  M. Estep, J. Kelly

                                                                                                        October 9th:           Presenters;  S. Pradhan, K. Mann
                                                                                                        October 23rd:        Presenters;  B. McCloud

We will also have a quick presentation of Websites

                                                                                                        November 6th:      Presenters;  J. Janson, U. Vatas        
November 20th:    Presenters;  D. Donohue, A Birerdinc
                                                                                                        December 4th:      Presenters;  S Dols, D. Shahan

Each week of the eight weeks we will meet, two students will present an historical/Landmark  paper from the literature which introduces a methodology that revolutionizes a field of Biology, and a recent paper indicating how this technique has changed or how it is still in use.

Select a paper from the following list and then it is up to you to find the relevant recent article that uses this technique or indicates the evolution of the technique. Once you have both references, email me the links so I can place them on the site for the other students to collect.  If there is a particular technique that you are most interested in which is not listed below, please email me so I can add it to the list for the other students.

Molecular Cell Biology:  Lodish, Berk, Zipursky, Matsudaira, Baltimore and Darnell on NCBI

Each student will present a 30-45 min Powerpoint presentation followed by questions.
Students are graded on their performance in class both a presenter and participant.  
Each student will complete a 3-4 page summary of their chosen papers on the last day of class December 4th.


Such examples are as follows:



Recombinant DNA

* To be presented September 25th by M. Estep

Electrophysiology

            * Will  be presented by J. Kelly,  Sept 25th

Historical Paper
Improved Patch-Clamp techniques for high resolution current recording from cells and cell free membrane patches.

New Paper
Protofibrils of amyloid Beta-protein inhibit specific K+ currents in neocortical cultures
Ye, Chian P. et al
Neurobiology of Disease 13 (2003) 177-190

PCR

            * subject selected, S. Pradhan and will be presented October 9th

Historical paper: 




 
 
 
 
 
 
 
 
 
 
 
 
Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction




 
 
 
 
 
 
 
 
 
 
 
 
Mullis K. B; Faloona F. A; Scharf S; Saiki R. K; Horn G; Erlich H. A.1986




 
 
 
 
 
 
 
 
 
 
 
 
Following is the URL for this paper: http://sunsite.berkeley.edu/cgi-bin/ebind2html/pcr/004




 
 
 
 
 
 
 
 
 
 
 
 





 
 
 
 
 
 
 
 
 
 
 
 
New paper:




 
 
 
 
 
 
 
 
 
 
 
 
Early diagnosis of SARS Coronavirus infection by real time RT-PCR 




 
 
 
 
 
 
 
 
 
 
 
 
Leo L.M Poon; Kwok Hung Chan; On Kei Wong; Wing Cheong Yam; Kwok Yung Yuen; Yi Guan; Y.M. Dennis Lo; Joseph S.M. Peiris Journal of clinical virology 28, (2003), 233-238




 
 
 
 
 
 
 
 
 
 
 
 
This paper is available through Elsevier

In Situ Hybridization

            * subject selected, K. Mann and will be presented October 9th

Brauth, SE, Tang, Y, Liang, W, and Roberts TF. (2003) Contact call-driven zenk mRNA expression in the brain of the budgerigar (Melopsittacus undulatus). Molecular Brain Research. 117 , 97-103.

    Molecular Brain Research is available through Mason E-journals.

Gall, JG, and Pardue, M. (1969) Formation and detection of RNA-DNA hybrid molecules in cytological preparation.  Proceedings of the National Academy of Sciences of the USA, 63 (2), 378-383.

    Available through JSTOR.

Microarray Analysis

             * Subject selected - B. McCloud and will be presented on October 23rd

Historical paper

Krakow D, Sebald ET, Pogue R, Rimoin LP, King L, Cohn DH.

Analysis of clones from a human cartilage cDNA library provides insight into
chondrocyte gene expression and identifies novel candidate genes for the
osteochondrodysplasias. Mol Genet Metab. 2003  May; 79(1):34-42. Available Online on GMU Ejournals.

Gene Therapy

              * Subject selected -J. Janson and will be presented on November 6th

                      Historical paper

Correction of Adenosine Deaminase Deficiency in Cultured Human T and B Cells by

Retrovirus-Mediated Gene Transfer

Cloning - Hello Dolly


            * Subject selected -U. Vatas and will be presented on November 6th

                      Historical paper

Wilmut, I.; Schnieke, A. E.; McWhir, J.; Kind, A. J.; Campbell, K. H. S.  Viable offspring derived from fetal and adult mammalian cells    (1997) Nature, 385(6619) pp 810-813
http://gateway1.ovid.com/ovidweb.cgi#11

DNA sequencing & SNP


             *Subject selected -A. Birerdinc and will be presented on November 20th

Historical Paper

Maxam AM, Gilbert W.
A new method for sequencing DNA.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4.
PMID: 265521 [PubMed - indexed for MEDLINE]

Zegura SL, Karafet TM, Zhivotovsky LA, Hammer MF. Links
High Resolution SNPs and Microsatellite Haplotypes Point to a Single, Recent Entry of Native American Y Chromosomes into the Americas.
Mol Biol Evol. 2003 Oct 31 [Epub ahead of print]
PMID: 14595095 [PubMed - as supplied by publisher]

Capturing of neuronal 3D structure in digital form


             *Subject selected -D. Donohue and will be presented on November 20th